Regulatory

Part:BBa_K310017

Designed by: Erin Borchardt   Group: iGEM10_UIUC-Illinois   (2010-10-27)

gadX 3'UTR (gadY target)

Targeted Regulation by sRNAs:micF, micA, and gadY. sRNAs expressed under lac promoter.
Comparison of target gene activation between gadY/gadX and key1/lock1 (J01008/J01010). gadY and key1 expressed under lac promoter

This part is the 3'UTR of the gadX mRNA. It contains the target sequence for gadY sRNA. Added to a protein coding sequence, the gadX 3'UTR will destabalize the mRNA transcript resulting in low expression. Upon the expression of gadY sRNA, the sRNA-mRNA interaction will stabalize the transcript resulting in activated gene expression.

Usage and Biology

Use this part in conjunction to gadY sRNA (BBa_K310001). See gadY sRNA page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None